Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential

In a recent study by Prof. Barboni, Prof. Russo and their team, the handheld cold atmospheric plasma, piezobrush® PZ2 (Relyon Plasma, Germany) was utilised in treatment of electrospun PLGA with highly aligned microfibers. The physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs) cultured on plasma treated surface were investigated.  

Applying variable treatment for exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm), it has been proved that utilising PZ2 significantly improved the hydrophilic properties of the treated materials as a result of the incorporation of new oxygen polar functionalities on the microfibers’ surface. It has been shown that increasing the exposure time of treatment and decreasing the working distance play major role in optimising the results of surface treatment.  

In this case, plasma treatment not only did not alter the ovine amniotic epithelial cells’ biocompatibility but enhanced cell adhesion and infiltration onto the microfibers.  

The tenogenic lineage expressing tenomodulin, in the cell’s cytoplasm is reported as an indication of effective plasma treatment, comparing cells cultured on the treated and untreated microfiber.   

In general, applying PZ2 in plasma treatment of PLGA microfibers improved the activation of PLGA surface by improving the hydrophilicity and cell bio-responsiveness.  

Read the full text:  https://lnkd.in/dSaMmQR 

Vist our PZ2 product information page here

Reference: Mohammad El Khatib, Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential, Molecules 2020.